Molecular Detection of Pophyromonas gingivalis and Some Interleukins Related with Periodontal Inflammatory Diseases

Abstract

Natheer Ahmad Hameed*, Reyam Abdul Khuder Mohammed, Thaar Mohammed Najim and Mustafa Salah Hasan

In this study, a total of 50 abscess swabs were collected from patients suffering from periodontal inflammatory diseases, the result showed that, out of 50 specimens, 33(66%) were positive culture on different types of media and 17(34%) were related to other types of causative agents (no growth on culture media). According to positive cultures, gram stain, biochemical tests and Vitek system, 9(27.2%) Pophyromonas gingivalis out of 33 specimens was considered main types of bacterial isolates were related with periodontal inflammatory diseases, while 24(72.2%) was related to other types of bacteria. Molecular detection of specific 16srRNA gene of Pophyromonas gingivalis were done in all Pophyromonas gingivalis isolates from periodontal inflammatory diseases samples, the results showed that, all of 9(100%) of Pophyromonas gingivalis isolates were gave positive results for this gene by the presence of (153bp) bands when compared with allelic ladder. In addition, molecular study of specific 16srRNA gene of IL-6 was done for all 9 isolates that previously detected as Bifidobacterium. Polymerase chain reaction is a sensitive and specific method for identification of this gene. The results showed that, all 9(100%) Pophyromonas gingivalis isolates were gave positive results for this gene by the presence of (174bp) bands when compared with allelic. However, molecular detection of specific 16srRNA gene of IL-10 were done in all Pophyromonas gingivalis isolates from periodontal inflammatory diseases, samples, the results showed that, all of 9(100%) of Pophyromonas gingivalis isolates were gave positive results for this gene by the presence of (627bp) bands when compared with allelic ladder. The mean differences IL-6 and IL-10 levels between study groups including (patients with periodontal inflammatory diseases and control group) were significant increase (P≤ 0.05) in IL-6 and IL-10 in patients with periodontal inflammatory diseases compared with control group.